TruScript Core H Minus cDNA合成预混液提供了一种方便的单管cDNA合成流程，针对两步RT-qPCR应用进行了优化。该试剂盒包含一种双链特异性DNase（dsDNase），可在2分钟内去除RNA样品中的基因组DNA污染，而不影响RNA的质量或数量。它对双链DNA具有特定活性，确保RNA和单链DNA（如cDNA和引物）保持完整，使经过dsDNase处理的RNA可直接用于反转录反应。预混液含有TruScript Core H Minus逆转录酶（RT）和RNase抑制剂。RT酶具有高热稳定性、强韧性，并提高cDNA合成速率。RNase抑制剂可在高达55°C的温度下保护RNA不被降解。预混液还含有反应缓冲液、dNTPs、寡聚dT(18)和随机六聚体引物。它支持在42-55°C下，从1 pg到1 µg总RNA的可重复cDNA合成，并能在15-30分钟内完成反应。

TruScript Core H Minus cDNA Synthesis Master Mix offers a convenient one-tube workflow for cDNA synthesis, optimized for two-step RT-qPCR applications. The kit includes a double-strand specific DNase (dsDNase) that removes contaminating genomic DNA from RNA samples in 2 minutes without affecting RNA quality or quantity. Its specific activity towards double-stranded DNA ensures RNA and single-stranded DNA (like cDNA and primers) remain intact, allowing dsDNase-treated RNA to be directly used in reverse transcription reaction. The master mix contains TruScript Core H Minus Reverse Transcriptase (RT) and RNase Inhibitor. The RT enzyme, provides high thermostability, robustness, and increased cDNA synthesis rate. The RNase Inhibitor protects RNA from degradation at temperatures up to 55°C. The mix also contains reaction buffer, dNTPs, oligo(dT)18, and random hexamer primers. It supports reproducible cDNA synthesis from 1 pg to 1 µg of total RNA at 42-55°C, completing the reaction in 15-30 minutes.