TruScript Core H Minus RT Master Mix 提供了一种方便的一管法工作流程用于 cDNA 合成，优化用于两步 RT-qPCR 应用。它包含 TruScript Core H Minus 逆转录酶 (RT) 和 RNase 抑制剂。该逆转录酶具有高热稳定性、稳健性，并能提高 cDNA 合成速率。RNase 抑制剂在最高 55°C 下保护 RNA 不被降解。该混合物还包含反应缓冲液、dNTPs、寡聚 dT18 引物和随机六聚体引物。它支持在 42-55°C 从 1 pg 到 1 µg 总 RNA 重现性地合成 cDNA，且反应在 15-30 分钟内完成。

ruScript Core H Minus RT Master Mix offers a convenient one-tube workflow for cDNA synthesis, optimized for two-step RT-qPCR applications. It includes TruScript Core H Minus Reverse Transcriptase (RT) and RNase Inhibitor. The RT enzyme, provides high thermostability, robustness, and increased cDNA synthesis rate. The RNase Inhibitor protects RNA from degradation at temperatures up to 55°C. The mix also contains reaction buffer, dNTPs, oligo(dT)18, and random hexamer primers. It supports reproducible cDNA synthesis from 1 pg to 1 µg of total RNA at 42-55°C, completing the reaction in 15-30 minutes.