TruScript Pro H Minus RT 反转录预混液含 DNase，为 cDNA 合成提供了方便的一管式操作流程，优化用于两步 RT-qPCR 应用。该试剂盒包含一种双链特异性 DNase (dsDNase)，可在 2 分钟内去除 RNA 样品中的基因组 DNA 污染，而不影响 RNA 的质量或数量。其对双链 DNA 的特异性活性确保 RNA 和单链 DNA（如 cDNA 和引物）保持完整，使经过 dsDNase 处理的 RNA 可直接用于逆转录反应。该预混液包含 TruScript Pro H Minus 逆转录酶 (RT) 和 RNase 抑制剂。RT 酶具有高热稳定性、强稳健性及提高的 cDNA 合成速率。RNase 抑制剂可在高达 55°C 的温度下保护 RNA 不被降解。混合液还包含反应缓冲液、dNTP、oligo(dT)18 和随机六聚体引物。它支持在 50-60°C 下从 1 pg 到 2.5 µg 的总 RNA 中可重复性地合成 cDNA，并可在 15-30 分钟内完成反应。

TruScript Pro H Minus RT Master Mix with DNase offers a convenient one-tube workflow for cDNA synthesis, optimized for two-step RT-qPCR applications. The kit includes a double-strand specific DNase (dsDNase) that removes contaminating genomic DNA from RNA samples in 2 minutes without affecting RNA quality or quantity. Its specific activity towards double-stranded DNA ensures RNA and single-stranded DNA (like cDNA and primers) remain intact, allowing dsDNase-treated RNA to be directly used in reverse transcription reaction. The master mix contains TruScript Pro H Minus Reverse Transcriptase (RT) and RNase Inhibitor. The RT enzyme, provides high thermostability, robustness, and increased cDNA synthesis rate. The RNase Inhibitor protects RNA from degradation at temperatures up to 55°C. The mix also contains reaction buffer, dNTPs, oligo(dT)18, and random hexamer primers. It supports reproducible cDNA synthesis from 1 pg to 2.5 µg of total RNA at 50-60°C, completing the reaction in 15-30 minutes.