TruScript Pro H Minus RT Master Mix offers a convenient one-tube workflow for cDNA synthesis, optimized for two-step RT-qPCR applications. It includes TruScript Pro H Minus Reverse Transcriptase (RT) and RNase Inhibitor. The RT enzyme, provides high thermostability, robustness, and increased cDNA synthesis rate. The RNase Inhibitor protects RNA from degradation at temperatures up to 55°C. The mix also contains reaction buffer, dNTPs, oligo(dT)18, and random hexamer primers. It supports reproducible cDNA synthesis from 1 pg to 2.5 µg of total RNA at 50-60°C, completing the reaction in 15-30 minutes.

TruScript Pro H Minus RT Master Mix 提供了一种方便的单管 cDNA 合成工作流程，针对两步 RT-qPCR 应用进行了优化。它包含 TruScript Pro H Minus 逆转录酶 (RT) 和 RNase 抑制剂。该 RT 酶具有高热稳定性、强健性，并能提高 cDNA 合成速率。RNase 抑制剂在最高 55°C 下保护 RNA 不被降解。该混合物还包含反应缓冲液、dNTP、寡聚 dT(18) 引物和随机六聚体引物。它支持在 50-60°C 下从 1 pg 到 2.5 µg 的总 RNA 进行可重复的 cDNA 合成，反应可在 15-30 分钟内完成。