5' G ↓ T G C A C 3'
3' C A C G T ↑ G 5'

SnapDigest Alw44I 快速限制性内切酶识别 G^TGCAC 位点，使用通用SnapDigest缓冲液，在37°C时可在5-15分钟内实现最佳切割。 SnapDigest 酶可快速完成 质粒、基因组、病毒 DNA 和 PCR 产物 消化, 无星活性, 在通用SnapDigest和SnapDigest Green缓冲液中表现出 100% 的活性。同一管中可同时使用多种酶进行消化，无需顺序步骤。常见的下游酶（如连接酶等）在这些缓冲液中也具有完全活性 。

 

SnapDigest Green缓冲液含有示踪染料（蓝色 ~3-5 kb，黄色 <10 bp 在 1% 琼脂糖凝胶中）和密度试剂，可直接用于凝胶上样。这些染料可能干扰荧光测量，建议在需要荧光分析的应用中使用标准 SnapDigest 缓冲液。

 

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产品优势

 

• 所有 SnapDigest 酶在通用型缓冲液中具有 100% 活性
• 缓冲液和下游应用的酶 100% 兼容
• 一般 5–15 分钟内即可完成酶切
• 可直接上样至凝胶
• 无信号活性

 

产品应用

 

• 分子克隆
• 限制位点作图
• 基因分型
• Southern 印迹
• 限制性片段长度多态性 (RFLP)
• SNP 分析

 

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