5' G G C C N N N N ↓ N G G C C 3'
3' C C G G N ↑ N N N N C C G G 5'

SnapDigest SfiI 快速限制性内切酶识别 GGCCNNNN^NGGCC 位点，使用SnapDigest缓冲液，50°C时在5-15分钟内实现最佳切割。 SnapDigest 酶可完成 质粒、基因组、病毒 DNA 和 PCR 产物 消化, 在通用SnapDigest和SnapDigest Green缓冲液中表现出 100% 活性。多种酶可同时进行消化。常见下游酶在缓冲液中有完全活性 。

 

SnapDigest Green缓冲液含有示踪染料和密度试剂，可直接用于凝胶上样。在荧光分析的应用中建议使用SnapDigest 缓冲液。

 

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产品优势

 

• 所有 SnapDigest 酶在通用型缓冲液中具有 100% 活性
• 缓冲液和下游应用的酶 100% 兼容
• 一般 5–15 分钟内即可完成酶切
• 可直接上样至凝胶
• 无信号活性

 

产品应用

 

• 分子克隆
• 限制位点作图
• 基因分型
• Southern 印迹
• 限制性片段长度多态性 (RFLP)
• SNP 分析

 

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