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GAPDH Loading Control Monoclonal Antibody (GA1R)
- 类型：内参抗体
- 品牌：Thermo Fisher
- 种属：Mouse,Rat,Human,Dog应用：WB,ICC/IF,IHC(P)抗体亚型：IgG1偶联物：Purified宿主：Mouse克隆号：GA1R浓度：1 mg/mL
规格: 100μg
货号: MA515738
价格: ¥3711.00
优惠: ¥2598.00
规格: 1mg
货号: MA515738-1MG
价格: ¥14438.00
优惠: ¥10107.00
应用 |
建议稀释比 |
|
|
免疫印迹 (WB) |
1:1,000-1:10,000 | |
|
免疫组化（石蜡） (IHC (P)) |
1:50-1:500 | |
|
免疫细胞化学 (ICC/IF) |
1:20-1:200 | |
| 流式细胞分析 (Flow) | 2 µg/test | |
|
酶联免疫吸附实验 (ELISA) |
Assay-dependent | |
产品详细信息
This antibody detects GAPDH from BL-21 bacteria, Sf9 insect, Saccharomyces cerevisiae (yeast), human, mouse, rat, rabbit, hamster, and chicken samples.
MA5-15738 has been successfully used in Western blot, ICC, IF, IHC (P), FACS and ELISA.
MA5-15738 may be stored at 4°C short term. Add 0.05% sodium azide if desired. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
靶标信息
GAPDH (Glyceraldehyde-3-phosphate dehydrogenase) is a catalytic enzyme commonly known to be involved in glycolysis. GAPDH exists as a tetramer of identical 37-kDa subunits and catalyzes the reversible reduction of 1,3-bisphosphoglycerate to glyceraldehyde 3-phosphophate in the presence of NADPH. Apart from playing a key role in glycolysis, GAPDH is ubiquitously expressed and displays other activities unrelated to its glycolytic function. GAPDH is reported to be involved in the processes of DNA replication, DNA repair, nuclear RNA export, membrane fusion and microtubule bundling. Studies provide evidence of GAPDH playing an essential part in gene expression observed in apoptosis and as part of the cellular phenotype of age-related neurodegenerative diseases. Further, GAPDH is involved in other cellular processes ranging from membrane fusion, and neuronal apoptosis in cancer. GAPDH is reported to bind to a variety of other proteins, including the amyloid precursor protein, mutations in which cause some forms of Alzheimer's disease (AD), and the polyglutamine tracts of Huntingtin, the protein product aberrant forms of which are causative of Huntington's disease. Associations between GAPDH, actin and tubulin have also be reported. Since GAPDH is expressed at high levels in most tissues, it is useful as protein loading control in Western Blot analysis.