BODIPY™ FL dye is a bright, green-fluorescent dye with a high extinction coefficient and fluorescence quantum yield. In contrast to the highly water soluble fluorophores Alexa Fluor™ 488 dye and fluorescein (FITC), BODIPY FL dyes have unique hydrophobic properties ideal for staining lipids, membranes, and other lipophilic compounds.

BODIPY™ FL 染料是一种明亮的绿色荧光染料，具有高消光系数和高荧光量子产率。相较于高水溶性荧光标记物Alexa Fluor™ 488染料和异硫氰酸荧光素（FITC），BODIPY FL染料具备独特的疏水性质，是脂质、细胞膜及其他亲脂性化合物染色的理想选择。

我们提供多种抗荧光染料抗体，可识别特定的荧光团，并且在大多数情况下能淬灭其荧光。这些抗染料抗体（包括我们的抗BODIPY™ FL荧光团抗体）可作为细胞非渗透性探针，用于确定荧光染料偶联的配体、蛋白质、细菌或其他生物分子是否已通过胞吞或胞饮过程被内化。

该抗BODIPY™ FL抗体（货号 A-5770）是针对BODIPY FL荧光团制备的。虽然它会与其他BODIPY染料发生不同程度的交叉反应，但与非BODIPY染料无明显交叉反应。在溶液检测中，我们发现该抗体能有效淬灭BODIPY FL荧光团的荧光，对BODIPY TR染料的淬灭程度较低，而对BODIPY™ TMR染料的淬灭效果不明显。

Invitrogen™采用灵敏的淬灭检测法，确保提供的该抗体始终具有高且稳定的效价值。根据认证，在100 mM磷酸钠缓冲液（pH 8.0）中进行检测时，20微升（µL）本品抗体溶液可淬灭1 mL浓度为50 nM的BODIPY FL染料溶液≥50%的最大荧光（即≥50%最大荧光淬灭效果）。BODIPY FL染料的最大淬灭效果约为游离染料荧光的80%。由于空间位阻，与蛋白质共价结合的BODIPY FL荧光团的最大荧光淬灭效果可能会显著降低。

建议操作： 使用前，将蛋白质偶联物溶液在微量离心机中短暂离心；随后仅取上清液加入实验。此步骤可消除储存期间可能形成的任何蛋白质聚集体，从而减少非特异性背景染色。

We offer a variety of anti-fluorescent dye antibodies that recognize specific fluorophores and, in most cases, quench their fluorescence. These anti-dye antibodies, including our antibody to the BODIPYTM FL fluorophore, can serve as cell-impermeant probes for determining whether fluorescent dye-conjugated ligands, proteins, bacteria, or other biomolecules have been internalized by endocytic or pinocytic processes.

The anti-BODIPYTM FL antibody (A-5770) was raised against the BODIPY FL fluorophore and exhibits no appreciable cross-reaction with non-BODIPY dyes, although it will cross-react to varying extents with other BODIPY dyes. In solution assays, we have found that this antibody effectively quenches the fluorescence of the BODIPY FL fluorophore, but quenches the BODIPY TR dye to a lesser extent and does not significantly quench the BODIPYTM TMR dye.

Invitrogen uses a sensitive quenching assay to ensure that this antibody is provided at a consistently high titer value. As supplied, 20 µL of the antibody solution is certified to produce ≥50% of the maximal fluorescence quenching of 1 mL of a 50 nM solution of BODIPY FL dye, assayed in 100 mM sodium phosphate, pH 8.0. Maximal quenching for BODIPY FL dye is ~80% of the fluorescence of the free dye. Due to steric hindrance, maximal fluorescence quenching of the BODIPY FL fluorophore covalently bound to protein may be significantly less. It is a good practice to centrifuge protein conjugate solutions briefly in a microcentrifuge before use; only the supernatant should then be added to the experiment. This step will eliminate any protein aggregates that may have formed during storage, thereby reducing nonspecific background staining.