检测灵敏度	150 pg/mL
检测范围	0.15-40 ng/mL
样本类型/体积	{Plasma=50 µL, Serum=50 µL, Supernatant=100 µL}
手工操作时间	1 hr 20 min
总时间	4 hr 45 min
均相（无需洗涤）	No
批间差异	<12%
批内差异	<10%
检测仪器	Absorbance Microplate Reader
产品规格	96 Tests
产品成分	Pre-coated 96 well plate Standard Assay Diluent concentrate Biotinylated Detection Antibody SAV-HRP Wash Buffer Chromogen Stop Solution Adhesive Plate Covers
运输条件	Wet ice
储存温度	Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C
蛋白名称	IL5RA
物种（Tested）	Human
检测试剂盒格式	Sandwich ELISA Kit
检测抗体结合物	Biotin
标签或染料	HRP

 

The Human Interleukin 5 Receptor Alpha/CD125 (Hu IL-5RA) ELISA quantitates Hu IL-5RA in human serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu IL-5RA.

 

原理方法

The Human IL-5RA solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.

 

严格验证

Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

 

靶标信息