优点：

- High yields of full length cDNA up to 20 kb
- Efficient cDNA synthesis at wide temperature range from 42°C to 65°C
- Increased synthesis rate—complete cDNA synthesis in 15-30 minutes
- High sensitivity and specificity

数据：

图1.Effective removal of genomic DNA using dsDNase

Two-step RT-qPCR analysis of PBGD gene with (RT+) and without (RT-) reverse transcriptase. cDNA synthesis was performed from 0.2ng total Jurkat RNA using Maxima First Strand cDNA Synthesis Kit with dsDNase (#K1671) or other kits including gDNA removal step. Flat RT-plot with Maxima kit indicates complete removal of contaminating gDNA, whereas RT-reactions with other suppliers' kits indicate amplification of residual gDNA.

图2.Highly sensitive two step RT-qPCR
A – amplification plot.
B – standard curve.
Amplification of the human PPP1CA gene was performed on varying amounts of Jurkat cell total RNA (5 μg, 2.5 μg, 1 μg, 100 ng, 10 ng, 1 ng, 100 pg, 10 pg, 1 pg). First strand cDNA was generated with the Maxima First Strand cDNA Synthesis Kit for RT-qPCR (#K1641). cDNA was amplified with the Maxima Probe/ROX qPCR Master Mix (2X) using the TaqMan assay specific for PPP1CA. Reactions were performed on an ABI® 7500 Real-Time PCR instrument. 1 pg of total RNA was successfully detected. Reaction efficiency was 105%, slope-3.29, R2=0.999.

图3.Reproducible RT-qPCR results using Maxima First Strand cDNA Synthesis Kit for RT-qPCR
First strand cDNA was generated from 100 ng to 1 pg of total Jurkat cell RNA with the Maxima First Strand cDNA Synthesis Kit for RT-qPCR (#K1641) in 16 replicate reactions. Synthesized cDNA was used as a template in subsequent qPCR with Maxima SYBR Green/ROX qPCR Master Mix (#K0221) on the ABI® 7500 Real-Time PCR instrument. Parallel RT reactions demonstrate reproducible cDNA synthesis and low variability levels with a wide range of starting RNA amounts.

图4.Greater yields and higher sensitivity using Maxima First Strand cDNA Synthesis Kit for RT-qPCR
Amplification of the E. coli 23S RNA gene was performed on 10-fold serial dilutions of total RNA (30 ng to 3 fg). First strand cDNA was generated using the Maxima First Strand cDNA Synthesis Kit for RT-qPCR (red) and a reverse transcription kit from another vendor (green). cDNA was amplified using Maxima SYBR Green/ROX qPCR Master Mix (#K0221) on the ABI® 7500 Real-Time PCR; instrument. All starting RNA amounts (30 ng to 3 fg) were successfully detected using the Maxima First Strand cDNA Synthesis Kit with lower Cq and 102% reaction efficiency.

图5.dsDNase treatment does not compromise RNA quality and quantity

Serial dilutions of total RNA were used in cDNA synthesis with Maxima First Strand cDNA Synthesis kits with and without dsDNase step. No changes in Cq values are observed between the compared protocols.

组成成分：

▪ Maxima Enzyme Mix
▪ 5X Reaction Mix
▪ nuclease-free water

相关产品：

▪ DNA 片段标准品

▪ DNA ladder

▪ DNA 聚合酶

▪ PCR

▪ DNA纯化

▪ RNA纯化