COS-7细胞是由CV-1细胞株通过带有编码野生型T抗原的起始失活突变的SV40病毒转化后获得，可编码表达野生型T抗原，所以该细胞适合作为需要SV40T抗原表达的载体的转染宿主。该细胞表达T抗原，允许SV40病毒的溶解性生长，支持40℃时温度敏感性A209病毒的复制，并支持起始区域缺陷的SV40突变体的复制。

 

细胞质控检测

1) 支原体检测结果：阴性
2) 细菌检测结果：阴性
3) 真菌检测结果：阴性

4) 种属鉴定结果：采用PCR引物扩增技术对该细胞株进行种属鉴定，结果显示为非洲绿猴细胞，且无其他常见物种细胞污染。

 

动物种别

非洲绿猴

 

组织来源

肾脏；经SV40转化

 

形态

成纤维细胞样，贴壁生长

 

培养基和添加剂配方

SV40转化的非洲绿猴肾细胞COS-7 完全培养液 配方：

• DMEM培养基 (PBM，货号609044)：90%
  
• 胎牛血清FBS (PBM，货号FBS141)：10%

 

参考传代条件

• 参考传代比例：1：4-1：8
• 参考传代周期：2-4天
• 参考换液频率：每周2-3次，传代时换液
• 冻存液配方：完全培养液95%，DMSO 5%
• 培养条件：气相：空气95%，二氧化碳5%；温度：37摄氏度。

 

细胞运输方式

活细胞保温运输或冻存管干冰运输。

 

供应限制

仅供研究之用

 

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