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53BP1 Recombinant Rabbit Monoclonal Antibody (7H14L12), Alexa Fluor™ Plus 555
- 类型：流式抗体
- 品牌：Thermo Fisher
- 种属：Human应用：Flow,ICC/IF抗体亚型：IgG偶联物：Alexa Fluor Plus 555/AFP555宿主：Rabbit克隆号：7H14L12浓度：1 mg/mL
规格: 20 μL
货号: 703580RP555XS
价格: ¥2400.00
优惠: ¥1680.00
规格: 50 μL
货号: 703580RP555
价格: ¥3890.00
优惠: ¥2723.00
|
应用 |
建议稀释比 |
|
|
免疫细胞化学 (ICC/IF) |
1:100 - 1:200 |
|
| 流式细胞分析 (Flow) |
0.06-0.5 µg/test |
|
产品详细信息
Alexa Fluor™ Plus recombinant antibodies are conjugated using new, proprietary dye chemistry so you can generate stunning data. Alexa Fluor™ Plus antibodies represent an advancement in fluorescent conjugate technology. Alexa Fluor™ Plus antibodies provide brighter signal compared to leading Alexa Fluor™ antibodies, providing you with better signal-to-noise for your critical experiments. These antibodies show better specificity and lot-to-lot consistency as these are recombinant antibodies, generated by cloning specific genes for the desired antibodies into an expression vector and expressed in vitro.
Using conjugate solutions: Centrifuge the protein conjugate solution briefly in a microcentrifuge before use; add only the supernatant to the experiment. This step will help eliminate any protein aggregates that may have formed during storage, thereby reducing nonspecific background staining.
Applications Tested: This 53BP1 antibody has been tested by immunocytochemistry and flow cytometric analysis of HeLa cells. This may be used for immunocytochemistry at 5 µg/mL and for flow cytometry at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Excitation: 553 nm; Emission: 568 nm; Laser: Yellow Laser
Filtration: 0.2 µm post-manufacturing filtered.
靶标信息
53BP1 (P53-binding protein 1) plays a critical role in tumor suppression and is a putative substrate of ATM kinase. Upon DNA damage, 53BP1 is phosphorylated and localizes to the presumptive sites of damage, specifically, double-strand breaks. 53BP1 may have a role in checkpoint signaling during mitosis, enhances TP53-mediated transcriptional activation, and participates in DNA repair by maintaining genomic stability.