应用	建议稀释比
流式细胞分析 (Flow)	5 µL (0.125 µg)/test
同型对照	Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), PE-Cyanine7, eBioscience™

 

产品详细信息

 

Description: The SN6 monoclonal antibody reacts with human CD105, also known as Endoglin. CD105, an approximately 90 kDa disulfide-linked homodimer is expressed by vascular endothelial cells and some bone marrow cells and activated macrophages. It is suggested that CD105 functions in adhesion and embryonic angiogenesis.

 

Applications Reported: This SN6 antibody has been reported for use in flow cytometric analysis.

 

Applications Tested: This SN6 antibody has been pre-titrated and tested by flow cytometric analysis of the U937 cell line. This can be used at 5 µL (0.125 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

 

Light sensitivity: This tandem dye is sensitive photo-induced oxidation. Please protect this vial and stained samples from light.

 

Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-822-49) (100 µL cell sample + 100 µL IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333-54) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.

 

Excitation: 488-561 nm; Emission: 775 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.

 

Filtration: 0.2 µm post-manufacturing filtered.

 

靶标信息

 

CD105 or Endoglin is a Type I transmembrane protein, which is highly expressed on human vascular endothelial cells. It exists on an O- and N-glycosylated homodimer. Up regulation of endoglin expression has been demonstrated in tumor vasculature and proliferating cells, suggesting that it is a proliferation associated endothelial marker. CD105 binds to TGF beta 1 and 3 with high affinity but not to TGF beta 2.