Overview

The Neuron Isolation Kit, mouse depletes all non-neuronal cells so that "untouched neurons" remain.

Background information

The Neuron Isolation Kit, mouse is an indirect magnetic labeling system for the isolation of untouched neurons from cell suspensions of mouse neural tissue. Non-neuronal cells like astrocytes, oligodendrocytes, microglia, endothelial cells, fibroblasts, except erythrocytes, are indirectly magnetically labeled by using biotin-conjugated antibodies specific for non-neuronal cells in combination with Anti-Biotin MicroBeads. Isolation of highly pure unlabeled neuronal cells is achieved by depletion of the magnetically labeled cells. The cell number and composition of the neuronal cell fraction differs according to the mouse age and the brain region used for cell isolation. The isolation of neurons has been tested with CD-1® mice aged from embryonic day 18 (E18) to adult. For optimal results, the Neural Tissue Dissociation Kit – Postnatal Neurons or the Adult Brain Dissociation Kit have been used prior to this kit.

 

相关产品：

 

 ▪ MS分选柱（货号#130-042-201）

 ▪ LS分选柱（货号#130-042-401）

 ▪ 磁力架（货号#130-042-303）

 ▪ MS分选磁极（货号#130-042-102）

 ▪ ImunoSep buffer（货号#604025/604050）

 

数据：

新生儿细胞     成年小鼠细胞   图1 从P2和9周龄CD-1®小鼠脑中分离神经元细胞。 用神经组织解离试剂盒对P2小鼠脑进行解离，出生后神经元和成年小鼠脑使用成人脑解离试剂盒、小鼠和大鼠。 用神经元分离试剂盒小鼠、LS柱和MidiMACS™分离器分离神经元。 非神经元细胞和星形胶质细胞特异性抗体抗ACSA-2-PE，少突胶质细胞特异性抗体抗O4-PE，小胶质细胞特异性抗体CD11b-FITC对分离的细胞进行荧光染色。 用MACS Quant™分析仪10对细胞进行流式细胞术分析。 基于散射信号和碘化丙啶荧光，排除细胞碎片和死细胞。