Overview

CD11b/c (Microglia) MicroBeads have been developed for the magnetic isolation of rat cells based on the expression of the CD11b and CD11c antigens.

Background information

The CD11b/c (Microglia) antibody appears to recognize a common epitope shared between CD11b and CD11c. CD11b, also known as integrin alpha M or Mac-1, is a component of the complement factor 3 (CR3), whereas CD11c, also called as

integrin alpha X, is a component of complement receptor 4 (CR4).

CD11b and CD11c are expressed on microglia, macrophages, monocytes, granulocytes, NK cells, and dendritic cells.

For optimal results, the Neural Tissue Dissociation Kit (P) (# 130‑092‑628) is required for the generation of a single-cell suspension.

 

相关产品：

 

 ▪ MS分选柱（货号#130-042-201）

 ▪ LS分选柱（货号#130-042-401）

 ▪ 磁力架（货号#130-042-303）

 ▪ MS分选磁极（货号#130-042-102）

 ▪ ImunoSep buffer（货号#604025/604050）

 

数据：

图1 用神经组织解剖试剂盒(P)、gentleMACS Dissociator、CD11b/c（小胶质细胞）微珠、MiniMACS™分离器和两个MS柱从出生后P3日大鼠脑组织中分离CD11b/c细胞。用CD11b/c-FITC（#130-105-273）与CD45-APC联合荧光染色，用MACSQuant分析仪进行流式细胞术分析。 基于散射信号和碘化丙啶荧光，排除细胞碎片和死细胞。