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Anti-O4 MicroBeads, human, mouse, rat

类型:细胞分选试剂盒
品牌:Miltenyi
种属:Mouse,Rat,Human

规格: for 1×10E9 total cells

货号: 130-094-543

价格: ¥9286.34

优惠: ¥7243.00

数量:

 

Overview


Anti-O4 MicroBeads have been developed for the positive selection or depletion of O4+ immature oligodendrocytes from single-cell suspensions. Anti-O4 Microbeads can be used with human, mouse or rat sample material.


Background information


During oligodendrocyte development, late A2B5+ oligodendrocyte progenitors start to express O4. As the cells differentiate, A2B5 expression ceases, whereas O4 expression persists. More mature cells start to synthesize galactocerebroside, MBP, PLP, MOG, and O1. In the peripheral nervous system, O4 expression is found on Schwann cells. Dissociated mouse brain tissue derived from P3–7 CD1 mice contains approximately 5–10% O4+ cells.

For the most efficient isolation of O4+ cells, P3–7 (postnatal day 3–7) rodents are recommended.

 

相关产品:

 

 ▪ MS分选柱(货号#130-042-201)

 ▪ LS分选柱(货号#130-042-401)

 ▪ 磁力架(货号#130-042-303)

 ▪ MS分选磁极(货号#130-042-102)

 ▪ ImunoSep buffer(货号#604025/604050)

 

 

数据:

 

 

1.JPG

 

图1 O4+ cells were isolated from P6 mouse brain tissue using the Neural Tissue Dissociation Kit (P), the gentleMACS™ Dissociator, FcR Blocking Reagent, Anti-O4 MicroBeads, a MiniMACS™ Separator, and two MS Columns. Cells were fluorescently stained with Anti-O4-PE prior to separation. Cells were analyzed using the MACSQuant® Analyzer.

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